Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. Label-free detection. 25 pM (0. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. plate type, read area and number of images per well, plus specify positive/negative wells. We show how the DuoLuc reporter assay and SpectraMax iD5 reader are used to detect nuclear factor-κB (NF-κB) activation in a mammalian cell model. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. 00. edu) to be added as a trained user in iLab. 4. Plate stacker and robot integration The SpectraMax 340PC384 reader can be integrated. The following document is intended. Your applications, your modes, your choice. For more information, please visit us at Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. 8. OPTICS Mirrored optics focus the light into the sample volume, and cutoff filters are used to. The M5e Reader is easily automated. SpectraMax M2, M2e;. D 49 7. 6 for further experiment, so I'm confused which i would choose for OD value determination. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. ”The SpectraMax 190 reader can be integrated with Molecular . F1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. SpectraMax L reader, SpectraMax M5/M5e reader What is the StakMax timing with SpectraMax Plate Readers? If using the StakMax and a SpectraMax reader together, the time specifications for the movement of the StakMax are as follows:. PURPOSE. Temperature Regulation. After the plate is read, data will appear in the plate. 3: The cuvette chamber. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. #655801. 40266 Email: drugdiscoverytech@lifetech. The dynamic range of the assay was approximately 5 orders of magnitude. The performance of these two modes is not comparable to dedicated luminescence or timeresolved fluorescence instruments, or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. Molecular Devices and can be performed only on an Molecular Devices plate reader. 9000-0756. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. Test up to 3. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Examples: “Press carefully to engage,” “ Do not press down. Comes from Lab closure Approx. SpectraMax M5 Multi-Mode Microplate Reader. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190. Serial number location on Series II and Multi-Mode plate readers. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. The following measurements are automatically made by SoftMax Pro Software: baseline noise, dark noise, optical alignment, wavelength accuracy, photometric linearity and wavelength precision. edu, 814-863-3503. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Shop all used microplate readers and parts from a variety of manufacturers. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. book Page 1 Wednesday, July 7, 2010 2:09 PM The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS control panel are shown in boxed Helvetica type. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with abs. The FlexStation 3 reader’s optics are designed to easily adapt to changes in assay requirements while maintaining the optimal performance of a single-mode reader. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. Here the delta mP was 336, and Z’ factor was 0. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. D 3 1. There is currently no charge for using this plate reader. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. 1. Please contact Tatiana to reserve the plate reader time: [email protected] Inc spectramax m5 plate reader for fluorescence Spectramax M5 Plate Reader For Fluorescence, supplied by Danaher Inc, used in various techniques. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . iD5. SpectraMax M5e Multi-Mode Microplate Reader. Please contact Tatiana to reserve the plate reader time: tnl1@psu. B. Certain functionalities in the table above requires specific configurations. When the user applies a set temperature of 37°C, the top is heated to 37. It was then read from the top and bottom on the SpectraMax iD3 reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. See our app note for details:. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 005) Photometric PrecisionThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 000). 2. SpectraMax® M5 Multi-Mode Microplate Re. com 4. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution for. After the plate is read, data will appear in the plate section: 11. FlexStation. 0 out of 5 $ 3,799. . The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. 11. The M5 can read volumes down to 2uL with the uMax Low Volume Plate. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orMolecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. Wide range of applications. With optimized reagents, option to operate the. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. 2 with 1000 cells. The cuvette must be in place when you read the plate. Plates were measured at 450 nm with 650 nm as a reference (Molecular Devices, SpectraMax M). ELISA Plate Readers / Microplate Readers. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 2. 200 ‚ 1000 nm. series measured in a 96-well plate in the SpectraMax M5 and the Analyst GT. SpectraMax M5 reader SpectraMax M5e. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 03 in x 3. Add to Cart. Where is the instrument serial number located? The serial. eu@moldev. Our absorbance plate readers feature our PathCheck Sensor technology and. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Products : SpectraMax M2/M2e reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax 190 reader, VersaMax reader, Gemini EM reader, SpectraMax Plus 384 reader. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch the mode to absorbance cuvette (thus the emission wavelength display disappears). SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 VAL014, right table) assays on the SpectraMax M5, M5e, or FlexStation 3 reader are shown below. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Molecular Devices. In the M2, M5 and Plus. 2. 2. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Clear Filters. 8. 96/384 - For the SpectraMax 340PC384, press to select to read a 96-well plate or a 384- well plate. Bioz Stars score: 86/100, based on 1 PubMed citations. Microplate readers’ standard detection modes. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and wester. The SpectraMax M3 Microplate Reader is supplied with SoftMax Pro Data Acquisition and Analysis Software, Molecular Devices' industry leading all-in-one software. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. HTRF-compatible SpectraMax Multi-Mode Readers. Figure 2-23: Bottle holder To install the bottle holder: 1. We specialize in many of the Molecular Devices microplate readers, washers, and stackers including the SpectraMax®, VersaMax™, Gemini™, FlexStation™, AquaMax®, and StackMax® lines. ". 312 gain calibration failed. The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. Experimental details and customization of the group table. Detection of pro-inflammatory factorsThe SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Specifications Plate mixing modes Selectable: off, once prior to any reading, and once prior to and between kinetic readings Plate mixing duration Selectable: 0 to 999 seconds (three-second default. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. We would like to show you a description here but the site won’t allow us. The entry should be SpectraMax GEMINI EM. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. A standard curve was plotted using a 4-parametric. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. FlexStation II. 筛选时,SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机,允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长,而无需额外再选购滤光片配件。这篇应FlexStation® 3 Multi-Mode Microplate Reader. One plate carrier adapter is. 000 to 2. Read More. Manufacturer: Molecular Devices. We would like to show you a description here but the site won’t allow us. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Fill a clean plate with water. columns 1, 3, 5, 7, 9 and 11 were seeded with 200,000 mock-transfected cells per well. Wide range of applications The VersaMax reader covers a wide range of applications:Molecular Devices. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Align the two slots on the bottle holder with the two pegs on the rail. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. 0 out of 5 $ 9,995 $ 9,495. Third this recommendation. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Supplier: Molecular Devices. Molecular devices SpectraMax M5e Pdf User Manuals. D 1 1. SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです! NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Robust, high-value microplate readers that can run samples based on pre-defined protocols and. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Wide range of applications. It features a modular design that allows users to add or change detection cartridges according to their needs. Optics. Perkin Elmer 1420-2560 Victor X Series Plate Reader Sipper. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. Set up Identify Acquire Analyze Imaging with the SpectraMax MiniMax Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3 System. easy to use and read multiple plates very quickly. 2. TR-FRET assay. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. 4. ScanLater Western Blot Detection Cartridge. 3: The cuvette chamber. The computer controlling the plate reader runs Windows XP. Based on the SpectraMax M5e platform, this five-mode reader addresses multiple application technologies including: absorbance, fluorescenceMolecular Devices | Life Science Applications | Bioanalytical. Be sure to insert . Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orThe Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader and computer with preload software (StakMax Microplate Handling System/racks not included). The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Thank you!. com 10. The M5 can read volumes down to 2uL with the uMax Low Volume Plate. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. 2. Dynamic Range. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. All SpectraMax® M series multi-mode microplate readers measure UV and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. SpectraMax M5 Plate Reader SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. The guide also covers the software features, the specifications, and the accessories of the systems. 4. SpectraMax Paradigm Multi-Mode Safety Guide SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. , contact. ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. edu The content of this site is published by the site owner(s) and. There is currently no charge for using this plate reader. measure the depth (optical pathlength) of samples with no temperature. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. TR-FRET assay principle. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Spectramax M5. The dual monochromator optics allow. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. There is currently no charge for using this plate reader. Description 1. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orSPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. For SpectraMax L or LMax II, LMax II. SpectraMax® M5 Microplate Reader Molecular Devices LLC. The calculated LOQ values were 0. Microplate Reader Shelf. The chamber. The plate is set up for reading and images are acquired according to specified parameters. 306 plate air cal fail,low light. See SpectraMax® Microplate Reader User Manual Download Page. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. 6 explains the lower values on the LmaxII384 plate reader. Be sure to insert the purple (SpectraMax M5 or M5e reader) or black (FlexStation 3 reader) plate adapter in the plate carriage prior to Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Standard curves were plotted using SoftMax® Pro Software (Figure 1). Supported Plates and Cuvettes. Expanded wavelength coverageDanaher Inc fluorescence plate reader spectramax m5 micro plate reader Fluorescence Plate Reader Spectramax M5 Micro Plate Reader, supplied by Danaher Inc, used in various techniques. We would like to show you a description here but the site won’t allow us. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. Thermo Scientific Microplate Reader Verification Plates Cat# 24072800. Self Service Available. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Microplate Readers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. The substrate solution (100 μL/well) was subsequently employed for 5-min reaction and the absorbance at 370 nm was monitored on a SpectraMax M5 plate reader (Molecular Devices). Information on how to reserve the plate readerThe GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. 2. For prep samples treated with the ELISA kit reagents, it is fast and user friendly. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). 8. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. Parts Only. A dialog box will appear, requesting you to confirm that the read is a pre-read. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. . Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. * Nunc. FL1 Validation Plate automatically validates the performance of the reader. Choose from a three- (M3), four- (M4), orThe SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. Contact Local Team. readers, the vertical light beam results in a pathlength that depends on the vol-. 2. Weighs about - 23 lbs. EarlyTox Caspase-3/7 R110 Assay. After the plate is read, data will appear in the plate. Data analysis Standard curves were plotted using the quadratic curve fit in SoftMax Pro Software, and the concentrations of cell lysates were calculated from the standard curves by the software. M5 E -HTRF Readers. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. Description 1. 12. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. The Molecular Devices Spectramax M5 Multi-Mode Plate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Find Molecular Devices SpectraMax M3/M4/M5/M5e Only Molecular Devices microplate plate readers offer the In need of a service manual for the SpectraMax M5 Find Molecular Devices SpectraMax Plus 384 – Forums, 190 – 1000 nm, tunable Molecular Devices SpectraMax Plus 384 Plate Reader. i3. Consider these features as well as all of the intended applications when choosing an appropriate luminescence reader for the lab. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. These robust readers have been placed in labs from Antarctica to. 0200-0729. Developed with Promega reagents to provide a. The M5 can read volumes down to 2uL with the. In this product video, see how the SpectraMax M5 quickly reads samples. Description. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. com 4. UV/Vis Photometric Performance: Wavelength Range. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Accordingly there are different configurations possible. 000000 out of 5. In the M2, M5 and Plus 384, the light. 1. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. 04 to 3. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Spectramax M5. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Gemini EM. Copia stocks a full line of used microplate readers. Click the OK button. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. We use Spectramax M5 microplate reader in our lab. For a higher . 025 fmol/well, 384-well plate) Quad monochromator: Fluorescein 2. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Temperature Regulation. 0 . Molecular Devices SpectraMax Plus 384. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . PERKIN ELMER Wallac Victor 2V Microplate Reader. BMG Nephelostar Laser-Based Microplate Nephelometer Microplate Reader. However, when a specific probe is not available, or it causes off-target effects on the. MICROPLATE READER: Used, in good condition, working microplate reader. Extended linear dynamic range. e. The SpectraMax M5 Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. View the Molecular Devices website. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. 7. 2 Software (multiple versions available) Windows 7 PC 60 day warranty All necessary communication electrical cables The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. 99. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. 40266 Email: drugdiscoverytech@lifetech. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Then the plate was returned to the. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. SpectraMax® microplate readers: reader check and error codes Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader,. spr SpectraMax Plus 384. 00 USD. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Plate section without PathCheck applied. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Step 4. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Designed to provide the simplicity, flexibility and power required for advanced data analysis. 1. It does not work as expected. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. Recertification is recommended every two years. SpectraMax M 系列多功能微孔板读板机(酶标仪)可测定紫外光 (UV) 和可见光 光吸收、荧光、发光、荧光偏振、时间分辨荧光 (TRF) 和均相时间分辨荧光 (HTRF) 。. No accessories are needed and SoftMax® Pro Software. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. spr * SpectraMax i3(x) SpectraTest ABS2 v1. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. To update the driver. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Information on how to. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. The drawing shows a standard microplate of 15 mm height covered with a lid. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter.